PCR Supplies
Meridian Life Science Gly-Free DNA Pol I Klenow exo-, 1ML
Glycerol-Free DNA Pol I Klenow Fragment exo- (HC) is a high concentration enzyme (50 U/uL) has mutations (D355A, E357A) which eliminates the 3’-5’ exonuclease activity. It is designed for dA and dT tailing for adapter ligation in next generation sequencing library preparation. The enzyme displays moderate strand displacement activity and can also be used to generate probes using random primers, dideoxy sequencing and random priming labelling. Glycerol-Free DNA Pol I Klenow Fragment exo- (HC) is in a glycerol-free storage buffer and is supplied with a Lyo-Ready Klenow Reaction Buffer and has been optimized to deliver excellent, stable performance without glycerol, allowing you to deliver reliable results with the added capability for lyophilization.
As One International Inc Hot Start Taq Polymerase 5U/ul, 10x Combination Buffer, Magnesium free, 10,000U
AS ONE HS Taq DNA Polymerase is a hot start polymerase designed to diminish the formation of non-specific priming events during reaction set up and the first ramp of thermal cycling. HS Taq features high specificity and sensitivity, greater yield compared to standard DNA polymerases. HS Taq enables the detection of low abundance tragets. HS Taq Polymerase includes magnesium free 10x Combination Buffer.
Genscript Corporation T7 RNA Polymerase
Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA polymerase with strict specificity for the T7 phage promoter The enzyme is widely used for the synthesis of specific transcripts from DNA in the 5 3 direction as well as being a suitable model for studying the mechanisms of transcription The RNA produced by T7 RNA Polymerase is suitable for many downstream applications GenScript is offering T7 RNA Polymerase produced by expression in an E coli strain carrying a plasmid encoding the T7 RNA Polymerase
As One International Inc Red-Taq DNA Polymerase 1.1x Master Mix, 2.0 mM MgCl2 (final concentration), 5,000Rxn
AS ONE Taq DNA Polymerase with Red Dye Master Mix is a ready-to-use 1.1x reaction mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications. AS ONE Taq Polymerase, the NH4+ buffer system, dNTPs, and magnesium chloride are conveniently present in the Taq DNA Polymerase Mix. An inert red dye and a stabilizer are also present to allow direct loading of the final products onto a gel for analysis. AS ONE Taq DNA Polymerase with Red Dye Mix offers several advantages. Set up time is significantly reduced. There is no need to buy and use separate loading dyes to load reaction products onto agarose gels for electrophoresis and subsequent visualization. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time.
As One International Inc Taq DNA Polymerase 5 U/ul 10x Ammonium Buffer, Magnesium free and Tween free, 5,000U
AS ONE Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa. AS ONE Taq DNA Polymerase has both a 5 prime to 3 prime DNA polymerase and a 5 prime to 3 prime' exonuclease activity. The enzyme lacks a 3 prime to 5 prime exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A overhang, which makes the enzyme ideal for TA cloning. Included with Taq is Tween/magnesium free 10x Ammonium Buffer.
As One International Inc Taq DNA Polymerase 5 U/ul glycerol free, 10x Ammonium Buffer and 10x Standard Buffer both detergent free, 5,000U
Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa. Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Glycerol free Taq is ideal for automation and free drying applications. Included with Taq are Tween/Triton free 10x Ammonium Buffer and 10x Standard Buffer.
Meridian Life Science Bst DNA Polymerase, 10ML
Bst DNA Polymerase is an enzyme derived from the large fragment of Bacillus stearothermophilus DNA Polymerase I. It contains 5´- 3´ DNA polymerase activity and strong strand displacement activity but lacks 5´- 3´ exonuclease activity. The strong strand displacement activity enables Bst DNA Polymerase to synthesize DNA at a constant temperature making it an ideal enzyme for isothermal amplification, including HDA, MCA, and Loop-Mediated Isothermal DNA Amplification (LAMP). It is supplied with an Enzyme Dilution Buffer and a Bst Reaction Buffer formulated to deliver the fastest amplification speed (time-to-results), yield, salt tolerance and sensitivity, speeding up time for optimization. For the most difficult templates, an Inhibitor-Tolerant Bst Buffer is available that is specifically designed for high performance when using crude samples such as sputum or saliva.
As One International Inc Taq DNA Polymerase 5 U/ul glycerol free, 10x Ammonium Buffer and 10x Standard Buffer, 500U
Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa. Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Glycerol free Taq is ideal for automation and free drying applications. Included with Taq are 10x Ammonium Buffer and 10x Standard Buffer.
As One International Inc Red-Taq DNA Polymerase 1.1x Master Mix, 2.0 mM MgCl2 (final concentration), 20,000Rxn
AS ONE Taq DNA Polymerase with Red Dye Master Mix is a ready-to-use 1.1x reaction mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications. AS ONE Taq Polymerase, the NH4+ buffer system, dNTPs, and magnesium chloride are conveniently present in the Taq DNA Polymerase Mix. An inert red dye and a stabilizer are also present to allow direct loading of the final products onto a gel for analysis. AS ONE Taq DNA Polymerase with Red Dye Mix offers several advantages. Set up time is significantly reduced. There is no need to buy and use separate loading dyes to load reaction products onto agarose gels for electrophoresis and subsequent visualization. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time.
As One International Inc High Fidelity Taq Polymerase, 10x Ammonium Buffer, 2,500U
AS ONE PR Taq DNA Polymerase is a thermostable high fidelity DNA polymerase with proof reading ability. This feature enables accurate and reliable PCR. Besides a 5' to 3' polymerase activity, PR-Taq exhibits a 3' to 5' exonuclease activity that enables the enzyme to correct base pair mismatches. This results in PCR products with very few errors and blunt ends. PR Taq Polymerase includes 10x Ammonium Buffer.
As One International Inc Red-Taq DNA Polymerase 5 U/ul 10x Ammonium Buffer, Magnesium free, 2,500U
AS ONE Taq DNA Polymerase with red dye is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. Taq contains a red dye which provides easy and quick identification of reactions to which enzyme was added and allows confirmation of complete mixing. The inert dye has no effect on downstream processes. Taq with Red Dye is added directly to the reaction mix and is used in the same manner as standard Taq DNA Polymerase. AS ONE Taq DNA Polymerase with red dye has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity. Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Red Taq Polymerase includes magnesium free 10x Ammonium Buffer.
As One International Inc Taq DNA Polymerase 5 U/ul 10x Standard Buffer, 1,000U
Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa. Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Included with Taq is 10x Standard Buffer.
Meridian Life Science Glycerol-Free Taq HS High, 500ML
Supplied as a blend of high‑performance glycerol‑free Taq DNA Polymerase with a Taq hot-start antibody and is suitable for difficult, multiplex PCR that require fast amplification, whilst maintaining both high specificity and sensitivity.Glycerol-Free Taq HS High Conc. is ideal for PCR kit manufactures and PCR molecular test facilities, requiring large amounts of DNA polymerases. The highly concentrated Glycerol-Free Taq HS High Conc. allows for concentrated bulk production of PCR master mixes and the glycerol free format is useful when using automated pipetting robots and other applications where accurate pipetting of small volumes is crucial.
As One International Inc Red-Taq DNA Polymerase 5 U/ul 10x Ammonium Buffer and 10x Combination Buffer, 10,000U
AS ONE Taq DNA Polymerase with red dye is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. Taq contains a red dye which provides easy and quick identification of reactions to which enzyme was added and allows confirmation of complete mixing. The inert dye has no effect on downstream processes. Taq with Red Dye is added directly to the reaction mix and is used in the same manner as standard Taq DNA Polymerase. AS ONE Taq DNA Polymerase with red dye has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity. Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Red Taq Polymerase includes 10x Ammonium Buffer and 10x Combination Buffer.
As One International Inc Taq DNA Polymerase 5 U/ul, 10x Ammonium Buffer and 10x Standard Buffer, 500U
Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa. Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Included with Taq are 10x Ammonium Buffer and 10x Standard Buffer.